The former US president's case against a company founded by an ex-MI6 officer was thrown out.
Mrs Justice Steyn threw out the case against Orbis Business Intelligence, a consultancy founded by former MI6 officer Christopher Steele, last month.
She found that Mr Trump's compensation claim was "bound to fail".
In a new ruling she also ordered Mr Trump to pay Orbis's costs "of the entire claim".
The order, obtained by the PA news agency on Thursday, also said Mr Trump has made no attempt to bring an appeal.
Mr Steele, who previously ran the Secret Intelligence Service's Russia desk, was the author of the so-called Steele dossier.
This included uncorroborated intelligence claims, denied by Mr Trump, that the former US president had been "compromised" by the Russian security service, the FSB.
Trump sues someone, loses, wasted time and pays fine
Trump gets charged, loses, pays fine/legal penalties
Trump talks out of his ass, gets sued, loses court battle, pays the lawsuit
This is entertaining, more please, I want to see his life erode into a neverending cycle of paying out the people he's harmed and getting his time wasted with court cases until he finally dies
Ultimately seeing his empire collapse while he’s still alive is the best punishment he can ever receive. It hits his narcissism and insecurity directly and in a way he can’t cover over.